Morpho-anatomical characterization and DNA barcoding analysis of Pluchea indica (L.) Less.

Abstract. Wahyuni DK, Mukarromah SR, Rakhmad P, Ilham M, Rakashiwi GA, Indriati DT, Yoku BF, Purnobasuki H, Junairiah, Prasongsuk S. 2022. Morpho-anatomical characterizationand DNA barcoding analysisof Pluchea indica (L.) Less.. Biodiversitas 23: 4272-4282. Less. is a member of the Asteraceae family and popular as medicinal plant. It very important to authenticate plants using various markers confirm labeling reveal bioactive compounds for pharmaceutical research. This study aims characterize morpho-anatomical aspects P. indica. Three samples obtained from Taman Husada Graha Famili, Surabaya, Indonesia were used in this study. Morphological studies carried out descriptively, while paraffin method was anatomical observations. analysis performed by amplifying aligning rbcLand matKgenes. Morphologically, indicahas organs like general, with specific characteristics leaves flowers. has an organ similar that globally, only there are histological features on its leaves, including anomocytic stomata, glandular cells, multicellular trichomes. Alignment reconstruction phylogenetic tree showed sample closely related GenBank database. Based characters barcodes, plant under confirmed L. (Less).